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BS EN 16187:2015

BS EN 16187:2015

$142.49

Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after pre-column derivatisation

Published By Publication Date Number of Pages
BSI 2015 24
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This European Standard specifies a method for the determination of fumonisin B 1 (FB 1) and fumonisin B 2 (FB 2) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 112 µg/kg to 458 µg/kg for FB 1+FB 2, 89 µg/kg to 384 µg/kg for FB 1 and 22 µg/kg to 74 µg/kg for FB 2.

For further information on the validation, see Clause 8 and Annex B.

PDF Catalog

PDF Pages PDF Title
5 Foreword
6 1 Scope
2 Normative references
3 Principle
4 Reagents
8 Table 1 — Preparation of mixed FBs calibration solutions for HPLC
9 5 Apparatus
10 Table 2 — Gradient conditions
6 Procedure
6.1 Extraction
11 6.2 Immunoaffinity column cleanup
6.3 Spiking procedure
6.4 Derivatisation and HPLC determination
6.4.1 Automated pre-column derivatisation programme
12 6.4.2 HPLC injections
6.4.3 Peak identification
6.4.4 Determination
7 Calculation
13 8 Precision
8.1 General
8.2 Repeatability
14 8.3 Reproducibility
15 9 Test report
16 Annex A (informative) Typical chromatograms
Figure A.1 — Typical chromatogram of baby food (pre-cooked flours of diastased maize and tapioca) spiked with 250,0 µg/kg fumonisin B1 (FB1) and 62,5 µg/kg fumonisin B2 (FB2)
Figure A.2 — Typical chromatogram of baby food (biscuits containing wheat, barley, rye, rice and maize) naturally contaminated with 164,8 µg/kg FB1 and 35,6 µg/kg FB2
17 Annex B (informative) Precision data
Table B.1 — Precision and recovery data for fumonisins B1+B2 in baby food
18 Table B.2 — Precision and recovery data for fumonisin B1 in baby food
19 Table B.3 — Precision and recovery data for fumonisin B2 in baby food
20 Annex C (informative) Comparison between the method in this document and EN 14352:2004 and EN 13585:2001 on fumonisins in maize
Table C.1 — Comparison between the method in this document and EN 14352:2004 and EN 13585:2001
21 Bibliography

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BS EN 16187:2015
$142.49