🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BS EN 17881:2024

BS EN 17881:2024

$102.76

Food authenticity. DNA barcoding of bivalves and products derived from bivalves using a defined mitochondrial 16S rRNA gene segment

Published By Publication Date Number of Pages
BSI 2024 20
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Category:

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This document specifies a method for the taxonomic identification of a single bivalve or piece of bivalve to the genus or species level using DNA barcoding. It allows the identification of a large number of commercially important bivalve species. This method was validated on raw mussels. Laboratory experience indicates additional applicability to processed bivalve products, e.g. cold smoked, hot smoked, salted, frozen, cooked, fried, and deep-fried samples. The described method is usually unsuitable for the analysis of highly processed foods, e.g. tins of mussels, with highly degraded DNA where the fragment lengths are not sufficient for amplification of the target. Furthermore, it is not applicable for complex seafood products containing mixtures of two or more bivalve species. The identification of bivalve species is carried out by PCR amplification of a segment of the mitochondrial 16S rRNA gene, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
9 4 Symbols and abbreviations
5 Principle
6 Reagents and materials
10 7 Apparatus
8 Procedure
8.1 Sample preparation
8.2 DNA extraction
8.3 PCR
8.3.1 General
8.3.2 PCR setup
11 8.3.3 PCR controls
8.3.4 Thermal cycling
12 8.4 Evaluation of PCR products
8.5 Evaluation of the PCR results
13 9 Sequencing
9.1 Sequencing of PCR products
9.2 Evaluation of sequence data
9.3 Comparison of the sequence with GenBank®
9.3.1 General
14 9.3.2 Sequence comparison of 16S rRNA amplicon sequences with GenBank®
10 Interpretation of database query results
15 11 Validation status and performance criteria
16 12 Test report
18 Annex A (informative)Practical laboratory data for 16S rRNA barcoding of examplary bivalve species

Related products

BS EN 17881:2024
$102.76