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BS EN ISO 18363-4:2021

BS EN ISO 18363-4:2021

$142.49

Animal and vegetable fats and oils. Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS – Method using fast alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol by GC-MS/MS

Published By Publication Date Number of Pages
BSI 2021 32
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This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3?MCPD esters (bound 3?MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.

This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Reagents
5.1 General
5.2 Standard and reference compounds
11 5.3 Standard solutions
5.3.1 General
5.3.2 Stock solutions
5.3.3 Working solutions
12 5.4 Other reagents
13 5.5 Reagent solutions
6 Apparatus
6.4 Ultrasonic bath.
7 Sample and storage
7.1 Sampling
14 7.2 Preparation of the test sample
7.3 Storage conditions
8 Procedure
8.1 Test sample preparation
15 8.2 Preparation of the calibration curve
8.3 Gas chromatography and mass spectrometry settings
16 9 Expression of results
9.1 General
9.2 Quantification of 2-MCPD- and 3-MCPD esters
18 9.3 Quantification of glycidyl esters
20 9.4 Quality control
10 Notes
22 11 Precision
11.1 General
11.2 Repeatability
11.3 Between-day reproducibility
23 Annex A (normative) Supporting tables
25 Annex B (informative) Statistical results of the ISO collaborative study
29 Annex C (informative) Chromatograms
31 Bibliography

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BS EN ISO 18363-4:2021
$142.49