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BS ISO 18184:2019

BS ISO 18184:2019

$189.07

Textiles. Determination of antiviral activity of textile products

Published By Publication Date Number of Pages
BSI 2019 50
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This document specifies testing methods for the determination of the antiviral activity of the textile products against specified viruses. Due to the individual sensitivities, the results of one test virus cannot be transposed to other viruses.

The textile products include woven and knitted fabrics, fibres, yarns, braids, etc.

PDF Catalog

PDF Pages PDF Title
2 National foreword
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
11 4 Principle
5 Virus and host cell
6 Warning
7 Apparatus
14 8 Sterilization of apparatus
9 Reagent and medium
15 9.4 Formaldehyde solution.
9.6 Inactivated fetal bovine serum (FBS)
19 10 Preparation
10.1 Restoration of host cell from cryopreservation
20 10.2 Subculture of host cell
21 10.3 Cell culture for the infectious virus titre assay
10.4 Preparation for test virus
10.4.1 General
10.4.2 Influenza virus
22 10.4.3 Feline calicivirus
23 10.4.4 Infectivity titre of the test viruses
24 10.5 Preparation for test specimen
10.5.1 Control fabric
10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test
10.6.1 General
25 10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity
11 Test procedure
11.1 Preparation of specimen
11.2 Deposit of virus to the specimens
11.3 Contacting time
26 11.4 Wash-out of virus immediately after deposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension
13 Determination of the infectious titre
13.1 Plaque assay
27 13.2 TCID50 method
14 Calculation of infectivity titre
14.1 Plaque assay
14.2 TCID50 method
14.2.1 Behrens and Karber method
28 14.2.2 Example of calculation
29 14.3 Test result
14.3.1 Verification of this test
30 14.3.2 Calculation of antiviral activity value
15 Test report
31 Annex A (informative) Virus strains and host cells
32 Annex B (normative) Infectivity titre test: Plaque assay
34 Annex C (normative) Infectivity titre test: TCID50 method
35 Annex D (informative) Composition of media
38 Annex E (informative) Testing method using specific pathogen free (SPF) embryonated hen’s eggs
43 Annex F (informative) Antiviral efficacy — Antiviral performance of the products
44 Annex G (informative) Interlaboratory test result (1)
46 Annex H (informative) Interlaboratory test result (2)
49 Bibliography

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BS ISO 18184:2019
$189.07