BSI PD ISO/TR 22455:2021
$142.49
Nanotechnologies. High throughput screening method for nanoparticles toxicity using 3D model cells
| Published By | Publication Date | Number of Pages |
| BSI | 2021 | 32 |
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Background 4.1 General 4.2 Effects of optical properties of NPs on in vitro cell viability assays |
| 12 | 4.3 New assay platform for in vitro toxicity screening of NPs diminishing optical interference |
| 15 | 4.4 Characteristics of 3D model cells |
| 17 | 4.5 Cell viability in response to NPs assessed using 3D model cells on a pillar insert |
| 21 | 4.6 Cellular uptake of NPs using 3D model cells on a pillar insert |
| 24 | 4.7 Discussion of alternative strategies to evaluate in vitro toxicity testing of NPs |
| 25 | 5 Methods for cell viability screening of NPs using 3D-model cells 5.1 General 5.2 Cell culture 5.3 Preparation of the pillar insert for in vitro screening |
| 26 | 5.4 Encapsulation of cells on a micropillar chip to generate 3D-model cells 5.5 NPs sample preparation 5.6 Exposing 3D-model cells to NPs |
| 27 | 5.7 Cell viability analysis using a WST assay 5.8 Cell viability analysis using live-cell imaging |
| 29 | Bibliography |
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