{"id":409253,"date":"2024-10-20T05:34:40","date_gmt":"2024-10-20T05:34:40","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-176412022\/"},"modified":"2024-10-26T10:13:11","modified_gmt":"2024-10-26T10:13:11","slug":"bs-en-176412022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-176412022\/","title":{"rendered":"BS EN 17641:2022"},"content":{"rendered":"

This document describes a method using isotopically labelled standards for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS\/MS). A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in food intended for infants and young children (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts. The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The measuring range of each mycotoxin in these naturally contaminated and\/or spiked food samples were: – AFB1: 0,085 7 \u00b5g\/kg – 11,4 \u00b5g\/kg; – AFB2: 0,079 2 \u00b5g\/kg – 12,5 \u00b5g\/kg; – AFG1: 0,062 8 \u00b5g\/kg – 20,9 \u00b5g\/kg; – AFG2: 0,052 0 \u00b5g\/kg – 15,0 \u00b5g\/kg; – AFM1: 0,034 2 \u00b5g\/kg – 0,110 \u00b5g\/kg; – OTA: 0,448 \u00b5g\/kg – 17,2 \u00b5g\/kg; – DON: 45,2 \u00b5g\/kg – 743 \u00b5g\/kg; – ZEN: 9,57 \u00b5g\/kg – 131 \u00b5g\/kg; – T-2: 10,3 \u00b5g\/kg – 57,9 \u00b5g\/kg; – HT-2: 9,50 \u00b5g\/kg – 81,8 \u00b5g\/kg; – FB1: 31,1 \u00b5g\/kg – 4 260 \u00b5g\/kg; – FB2: 44,2 \u00b5g\/kg – 1 300 \u00b5g\/kg. The measuring ranges of the method for each mycotoxin\/matrix combination are given in Table 8.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
8<\/td>\n4 Principles
5 Reagents <\/td>\n<\/tr>\n
14<\/td>\n6 Apparatus and equipment <\/td>\n<\/tr>\n
16<\/td>\n7 Procedure
7.1 Preparation of the laboratory sample
7.2 Test portions weighing
7.2.1 Cereals, cereal-based products
7.2.2 Milk powders, nuts, spices, dried fruits
7.3 Spiking with ISTD solutions
7.4 Extraction
7.4.1 General procedure <\/td>\n<\/tr>\n
17<\/td>\n7.4.2 Without IAC clean-up
7.4.3 With IAC clean-up <\/td>\n<\/tr>\n
18<\/td>\n8 LC-MS\/MS analysis
8.1 Operating conditions
8.2 Injection sequence <\/td>\n<\/tr>\n
19<\/td>\n8.3 Data treatment
9 Calculations
9.1 Identification and confirmation
9.2 Calibration curve and measuring range <\/td>\n<\/tr>\n
20<\/td>\n9.3 Calculations <\/td>\n<\/tr>\n
21<\/td>\n10 Precision
10.1 General
10.2 Repeatability
10.3 Reproducibility <\/td>\n<\/tr>\n
24<\/td>\n11 Test report <\/td>\n<\/tr>\n
25<\/td>\nAnnex A (informative)Precision data <\/td>\n<\/tr>\n
41<\/td>\nAnnex B (informative)Overview of the sample preparation <\/td>\n<\/tr>\n
42<\/td>\nAnnex C (informative)Example conditions for suitable LC-MS\/MS systems
C.1 General
C.2 LC system settings examples
C.2.1 LC conditions 1 <\/td>\n<\/tr>\n
43<\/td>\nC.2.2 LC conditions 2
C.2.3 LC conditions 3 <\/td>\n<\/tr>\n
44<\/td>\nC.2.4 LC conditions 4 <\/td>\n<\/tr>\n
45<\/td>\nC.2.5 LC conditions 5
C.3 MS system settings examples
C.3.1 MS conditions 1 <\/td>\n<\/tr>\n
47<\/td>\nC.3.2 MS conditions 2 <\/td>\n<\/tr>\n
49<\/td>\nC.3.3 MS conditions 3 <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Multimethod for the determination of aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone by LC-MS\/MS<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n52<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":409262,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-409253","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/409253","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/409262"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=409253"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=409253"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=409253"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}