{"id":465003,"date":"2024-10-20T10:38:11","date_gmt":"2024-10-20T10:38:11","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-178992024\/"},"modified":"2024-10-26T19:37:24","modified_gmt":"2024-10-26T19:37:24","slug":"bs-en-178992024","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-178992024\/","title":{"rendered":"BS EN 17899:2024"},"content":{"rendered":"
This document describes a spectrophotometric method for determining the chlorophyll-a content corrected for phaeopigments as a measure of the amount of phytoplankton for all types of surface water including marine water. The determination limit is usually 2 \u00b5g\/l to 5 \u00b5g\/l and is calculated by each laboratory individually. It can be as low as 0,5 \u00b5g\/l using 2 l of sample (or even more) and a 50 mm cuvette. NOTE In some measurement programs like marine studies on time series data and ecological status\/classification no correction for phaeopigments is used and acidification is omitted, e.g. as recommended by OSPAR.<\/p>\n
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
8<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 4 Principle 5 Interferences 6 Reagents <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 7 Apparatus <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 8 Procedure 8.1 General 8.2 Sample pre-treatment 8.3 Filtration 8.4 Extraction <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 8.5 Measurement 9 Quality assurance 9.1 Blank values 9.2 pH after acidification 9.3 Wavelength setting <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 9.4 Ratio A\/A’ 10 Calculations <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 11 Expression of results 12 Test report <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | Annex A (informative)Performance data A.1 Interlaboratory trial study in the Netherlands: A.2 Validation interlaboratory trial in Germany: <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | Annex B (informative)Theoretical backgrounds B.1 Meaning of the determination of the chlorophyll-a content B.2 Acidifying extracts B.3 Calculation of Phaeopigment concentration <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | Annex C (informative)Control samples C.1 Introduction C.2 Surface water C.3 Frozen spinach extracts C.4 Freeze-dried kale <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | C.5 Algae culture <\/td>\n<\/tr>\n | ||||||
21<\/td>\n | Annex D (informative)SCOR-Unesco method for determination of chlorophyll in sea-water D.1 Measurement of chlorophyll a, b, c and d with SCOR-Unesco method using chromatic equations <\/td>\n<\/tr>\n | ||||||
22<\/td>\n | D.2 Performance characteristics and comparison of methods D.2.1 Validation study in France <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Water quality. Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality<\/b><\/p>\n |